課程介紹：

 

1.Culture of bacterial cells

media preparation, bacterial isolation, incubation and stocks

Objectives

In this experiment, we run the culture of microorganisms in order to understand the

general idea of sterile procedures and realize its importance in the bio-research.

Instruments: Reagents:

laminar flow bench

autoclaved machine

electric Bunsen burner

analytical balance

pH meter with 4.0 and 10.0 pH solutions

4°C refrigerator

ultra-low temperture freezer

orbital shaking Incubator

water bath set

pipet aid

pipetman

graduated cylinders

autoclavable bottles and tubes

sterile plates

1.5 mL eppendorf

inoculating loop

stir bar

Kimwipe

2. Measurement of protein concentration

蛋白質濃度測定

一、目的:

1. 了解蛋白質濃度測定的方法

2. 學習分光光度計 (Spectrophotometer) 的測定原理和實際操作

二、實驗器材

1. Spectrophotometer (分光光度計；BioTek, μQuant)

2. Pipetman (微量吸管)

3. 96 well plate (96 孔盤)

4. Eppendorf (微量離心管)

5. 200 μl tip (200 μl 微量吸管尖)

3. Mass diffusion in a micro-channel

Objectives

The purpose of this experiment is to studythe characteristics of flow mixing in a micro-channel and to study the relationshipsbetween diffusivity and the geometry of a micro-channel.

Instrumental apparatus

(1) CCD

(2) Fluorescent dye

(3) Microscope

(4) Fluorescent light source

(5) Syringe pump

(6) Computer

(7) Micro-channel 200 μm and 300 μm

4. Passive mixing in a micro-channel

Objectives

First, wewould like to study the mixing characteristics of a micro-scale mixing.Then, westudy theinfluence of geometric parameters on passive mixingof two fluids. Thereafter,we want to compare the convective effects created by geometric variations.

Experimental apparatus

(1) Olympusmicroscopewith CCD

(2) Dyeand fluorescent dye

(3) Syringepump

(4) Two needles

(5) Mercury arc lamp housing

(6) Filter

(7) Differently geometric parameters of the micro channels

(8) Personal computer

(9) Program of Digital Image process